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Related Experiment Videos

Changes in CTP:phosphatidate cytidylyltransferase activity during rabbit lung development.

K J Longmuir, J M Johnston

    Biochimica Et Biophysica Acta
    |December 5, 1980
    PubMed
    Summary

    The activity of CTP:phosphatidate cytidylyltransferase in rabbit lungs significantly increases after birth, coinciding with the development of pulmonary surfactant. This enzyme is crucial for lung maturation.

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    Area of Science:

    • Biochemistry
    • Developmental Biology
    • Pulmonary Medicine

    Background:

    • CTP:phosphatidate cytidylyltransferase is a key enzyme in phospholipid synthesis.
    • Pulmonary surfactant development is critical for neonatal respiratory adaptation.
    • Understanding enzyme activity during development aids in studying lung maturation.

    Purpose of the Study:

    • To measure the activity of CTP:phosphatidate cytidylyltransferase in rabbit lung and liver microsomes during fetal and neonatal development.
    • To investigate the enzyme's kinetic properties and cofactor requirements.
    • To correlate enzyme activity with pulmonary surfactant production.

    Main Methods:

    • Microsomal fractions were isolated from rabbit lung and liver at various developmental stages.

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  • Enzyme activity was assayed by measuring the formation of CDP- CTP:phosphatidate cytidylyltransferase.
  • Kinetic parameters and cofactor requirements (Mg2+, ATP) were determined.

    • Main Results:

    • Microsomal CTP:phosphatidate cytidylyltransferase activity in the lung remained constant until late gestation, then increased 2.4-fold by day 1 postpartum.
    • Liver enzyme activity showed no significant changes and was lower than in lung tissue.
    • Optimal activity required phosphatidate, CTP, and Mg2+, with ATP inhibiting CTP hydrolysis.

    Conclusions:

    • A significant increase in lung CTP:phosphatidate cytidylyltransferase activity occurs during the perinatal period.
    • This rise in enzyme activity is concurrent with the appearance of phosphatidylglycerol-containing pulmonary surfactant.
    • The findings highlight the enzyme's role in neonatal lung development and surfactant synthesis.