Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Multiple reversal potentials for responses to L-glutamic acid.

J M Wojtowicz, M Gysen, J F MacDonald

    Brain Research
    |May 25, 1981
    PubMed
    Summary

    This study determined reversal potentials for L-glutamic and kainic acid in mouse neurons, revealing complex responses and potassium conductance involvement. Findings challenge existing models of neuronal excitability.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Synergistic effects of diet and exercise on hippocampal function in chronically stressed mice.

    Neuroscience·2015
    Same author

    Furthering the understanding of silicate-substitution in α-tricalcium phosphate: an X-ray diffraction, X-ray fluorescence and solid-state nuclear magnetic resonance study.

    Acta biomaterialia·2013
    Same author

    Depletion of new neurons by image guided irradiation.

    Frontiers in neuroscience·2011
    Same author

    Hippocampal neurogenesis in food-storing red squirrels: the impact of age and spatial behavior.

    Genes, brain, and behavior·2010
    Same author

    Gentamicin.

    Canadian Medical Association journal·2010
    Same author

    Bilateral simultaneous tubal pregnancy.

    American journal of obstetrics and gynecology·2010

    Area of Science:

    • Neuroscience
    • Cellular Biology
    • Biophysics

    Background:

    • Neuronal excitability is crucial for brain function.
    • Understanding the ionic basis of neuronal responses is key to deciphering neural signaling.

    Purpose of the Study:

    • To determine the reversal potentials for L-glutamic and kainic acid in cultured fetal mouse neurons.
    • To investigate the ionic conductances underlying neuronal responses to these amino acids.

    Main Methods:

    • Dissociated cell culture of fetal mouse brain and spinal cord neurons.
    • Conventional intracellular recording techniques.
    • Pressure microperfusion of L-glutamic and kainic acid.
    • Direct measurement of reversal potentials by altering membrane potential.

    Main Results:

    • Reversal potentials for L-glutamic and kainic acid were measured in cultured neurons.
    • Observed reversal potentials differed from previous reports and theoretical predictions based solely on sodium conductance.
    • Multiple response phases with distinct reversal potentials were observed in some neurons.
    • Evidence for potassium conductance participation in neuronal responses was found.

    Conclusions:

    • Neuronal responses to L-glutamic and kainic acid involve complex ionic mechanisms beyond simple sodium conductance.
    • Potassium conductance plays a role in mediating these responses.
    • The findings necessitate a revision of current models of neuronal excitability in response to excitatory amino acids.

    Related Experiment Videos