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Investigations on the structure and morphogenesis of some arboviruses isolated in the U.S.S.R
Abstract:
The morphology and some stages of morphogenesis in suckling mouse brains of hitherto serologically unclassified viruses Kaspiy (LEIV-A-63), Zavashan (LEIV-Ap-6158), Artashat (LEIV-A-2366), and Paramushir (LEIV-C-2268), isolated in the U.S.S.R., of GM-710 virus isolated in Scotland, and of Sokuluk (LEIV-K-400) virus belonging to the genus Flavivirus (family Togaviridae) were studied. Virion sizes were determined and changes in infected cells described. Based on their structure and morphogenesis, the viruses Kaspiy, Artashat, Zavashan and Paramushir were referred to the family Bunyaviridae and GM-710 virus was referred to the genus Orbivirus (family Reoviridae).
Insights
Morphological studies classified four uncharacterized viruses as Bunyaviridae and one as Orbivirus. This research aids in understanding viral families and their structures.
Area of Science:
- Virology
- Microbiology
- Cell Biology
Background:
- Several viruses, including Kaspiy, Zavashan, Artashat, Paramushir, GM-710, and Sokuluk, were previously unclassified serologically.
- These viruses were isolated from different geographical locations, including the U.S.S.R. and Scotland.
Purpose of the Study:
- To determine the morphology and morphogenesis of six unclassified viruses.
- To classify these viruses into appropriate viral families based on structural and developmental characteristics.
Main Methods:
- Electron microscopy was used to study virion morphology and morphogenesis in infected suckling mouse brains.
- Morphological characteristics and changes in infected cells were analyzed to infer viral classification.
Main Results:
- Viruses Kaspiy, Artashat, Zavashan, and Paramushir exhibited characteristics consistent with the family Bunyaviridae.
- GM-710 virus was identified as belonging to the genus Orbivirus within the family Reoviridae.
- Sokuluk virus, previously noted as Flavivirus (family Togaviridae), was also studied for comparative morphology.
Conclusions:
- The structural and developmental studies provided a basis for reclassifying several previously uncharacterized viruses.
- This research contributes to the accurate taxonomic placement of viruses within established viral families, aiding in future research and diagnostics.