Related Experiment Video
Updated: Jul 23, 2026

Techniques for the Evolution of Robust Pentose-fermenting Yeast for Bioconversion of Lignocellulose to Ethanol
Published on: October 24, 2016
Metabolism of acetaldehyde and custers effect in the yeast
Abstract:
Brettanomyces abstinens growing on different initial glucose concentrations showed an anaerobic inhibition of fermentation. This Custers effect decreased as the initial glucose concentration in the medium increased. Two aldehyde dehydrogenases, one NAD+-linked and the other NADP+-linked were observed. The results suggest that the NAD+-linked enzyme is involved in the production of acetic acid and is repressed by glucose. The NADP+-linked enzyme seems to be a constitutive enzyme. Acetyl-CoA synthetase activity also was not greatly affected by the growth conditions. The results support the earlier hypothesis that the Clusters effect in Brettanomyces is provoked by the reduction of NAD+ in the conversion of acetaldehyde to acetic acid.
More Related Videos
12:47Workflow Based on the Combination of Isotopic Tracer Experiments to Investigate Microbial Metabolism of Multiple Nutrient Sources
Published on: January 22, 2018
07:38Saccharomyces cerevisiae Exponential Growth Kinetics in Batch Culture to Analyze Respiratory and Fermentative Metabolism
Published on: September 30, 2018
Related Concept Videos
Fates of Pyruvate
In aerobic organisms, pyruvate is metabolized via the citric acid cycle to produce reduced coenzymes NADH and FADH2. These coenzymes are then oxidized in the electron transport chain to produce ATP and, in the process, regenerate the NAD+ and FAD. As seen in some cell types and organisms, fermentation...
Aldehydes and Ketones with Alcohols: Hemiacetal Formation
Alkylation of β-Ketoester Enolates: Acetoacetic Ester Synthesis
Microbial Fermentation
Bioreactor Controls-III
Production of Alcohol