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Comparison of semiautomatic digitizer-tablet and simple point counting performance in morphometry
Virchows Archiv. B, Cell Pathology Including Molecular Pathology
|January 1, 1981
Summary
Simple point counting and digitizer tablets offer similar morphometric results for basal cell analysis. Point counting is more efficient and cost-effective for biological studies, despite digitizer precision.
Area of Science:
- Histology
- Morphometry
- Cell Biology
Background:
- Chonchal epithelium basal cells exhibit normal and pathological alterations.
- Accurate morphometric analysis is crucial for understanding cellular changes.
Purpose of the Study:
- Compare the efficiency of an opto-manual digitizer-tablet (MOP-AM 03) and point counting for basal cell analysis.
- Evaluate morphometric results, including cellular and nuclear profiles and basal width.
Main Methods:
- Analysis of drawings of basal cells from normal and pathologically altered chonchal epithelium.
- Estimation of cellular, nuclear, and nucleolar sectional profile areas.
- Measurement of cell basal width (attachment to basement membrane).
- Comparison between digitizer-tablet and point counting methods.
Main Results:
- Morphometric results from both methods were virtually identical.
- Point counting demonstrated slightly higher efficiency than the digitizer-tablet.
- Digitizer-tablet equipment is significantly more expensive than point counting tools.
Conclusions:
- Simple point counting is a cost-effective and efficient method for basal cell morphometry.
- The high precision of digitizer-tablets may not significantly impact biological studies due to inherent biological and sectioning variations.
- Point counting is recommended for routine morphometric analysis in such studies.