Adenylate cyclase activity in bovine and human corneal endothelium

Adenylate cyclase activity was measured in particulate fractions prepared from bovine and human corneal endothelium. Bovine enzyme activity was stimulated by fluoride, 5'-guanylylimidodiphosphate (GppNp) and beta-adrenergic agonists. Drug stimulation patterns of the human enzyme appeared qualitatively similar, but activities were generally higher than those of the bovine enzyme. This study demonstrates the existence of a receptor-mediated adenylate cyclase system in bovine and human corneal endothelium and suggests a possible role for cycli AMP in corneal endothelial physiology.