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Detection of Infectious Virus from Field-collected Mosquitoes by Vero Cell Culture Assay
Published on: June 9, 2011
Infection of Israeli culicoides with African horse sickness, blue tongue and akabane viruses
Abstract:
Type 9 African horse sickness virus and type 4 bluetongue virus multiplied to a high titre in an Israeli strain of Culicoides puncticollis after intrathoracic inoculation. Akabane virus persisted for at least 10 days in this midge after intrathoracic inoculation but with little evidence of virus multiplication. All 3 viruses failed to multiply in C. puncticollis after ingestion by the oral route and all were inactivated by 4 days post infection. Five other species of Israeli Culicoides supported multiplication of bluetongue virus after intrathoracic inoculation.
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