Stability of CNS bindings sites under various conditions

The effects of (1) method of sacrifice, (2) treatment of tissue and membranes, and (3) gavaging on neurotransmitter receptors were assessed. Most variables did not affect binding, but freezing of forebrain tissue decreased protein recovery and therefore estimates of binding sites per brain weight. Serotonin binding was especially sensitive to freezing to neurotoxicology is discussed.