The ontogeny of spinal cord monoamines and the post-decapitation reflex

The ontogeny of the post-decapitation reflex (PDR) was examined in neonatal systemic 6-hydroxydopamine (6-OHDA) treated rats. The PDR was absent in the youngest (5 day) and oldest (60 day) 6-OHDA treated rats but present with attenuated characteristics at around 15 and 20 days. The reappearance of the PDR in these rats closely paralleled age-related fluctuations in spinal cord noradrenaline (NE) which increased until 15 days, then precipitously declined, but did not correlate with spinal dopamine (DA) or serotonin (5-HT). The failure of the alpha noradrenergic blocking agents chlorpromazine, phenoxybenzamine, prazosin, or yohimbine to eliminate the vestigial PDR in the 15-20-day-old, 6-OHDA-treated rats indicates that this is probably not mediated by remaining, undamaged spinal NE neurons at this age. Neither is it likely to be mediated by spinal 5-HT neurons since inhibition of 5-HT synthesis had no effect upon the PDR latency in normal or neonatal 6-OHDA-treated, 17-day-old rats. In contrast to NE levels which increased with age in normal rats, spinal cord 5-HT and DA levels at least as high at 5 days of age as at adulthood.