Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Methionine degradation in Candida utilis.

J Benítez, J Delgado, A Alonso

    Folia Microbiologica
    |January 1, 1982
    PubMed
    Summary

    Cell-free extracts of C. utilis exhibited transamination activities with methionine and other amino acids. Ethionine-resistant mutants showed a transport defect, hindering methionine utilization.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Compartmentalization of ceramide signaling: physical foundations and biological effects.

    Journal of cellular physiology·2000
    Same author

    Oscillatory activity in entorhinal neurons and circuits. Mechanisms and function.

    Annals of the New York Academy of Sciences·2000
    Same author

    [VIGILA: a tool for the epidemiologic surveillance of transmissible diseases].

    Revista cubana de medicina tropical·2000
    Same author

    HLA-DR51 expression failure caused by a two-base deletion at exon 2 of a DRB5 null allele (DRB5*0110N) in a Spanish gypsy family.

    Tissue antigens·2000
    Same author

    Stenotrophomonas maltophilia D457R contains a cluster of genes from gram-positive bacteria involved in antibiotic and heavy metal resistance.

    Antimicrobial agents and chemotherapy·2000
    Same author

    A randomized field trial of ACINDES: a child-centered training model for children with chronic illnesses (asthma and epilepsy).

    Journal of urban health : bulletin of the New York Academy of Medicine·2000

    Area of Science:

    • Biochemistry
    • Microbiology
    • Molecular Biology

    Background:

    • * Candida utilis* is a yeast with known metabolic pathways.
    • * Ethionine resistance* in mutants can indicate specific genetic alterations.
    • * Methionine metabolism* is crucial for cellular functions.

    Purpose of the Study:

    • * To investigate transamination activities* in *C. utilis* cell-free extracts.
    • * To characterize methionine utilization* in ethionine-resistant mutants.
    • * To identify potential transport lesions* affecting methionine uptake.

    Main Methods:

    • * Incubation of cell-free extracts* with amino acids (methionine, aspartate, alanine) and oxo-acids.
    • * Analysis of transamination reactions* including glutamate-aspartate, alanine-glutamate, methionine-glutamate, methionine-alanine, and alanine-aspartate.
    • * Comparison of wild-type and mutant strains* to identify metabolic differences.

    Main Results:

    • * Demonstrated various transamination activities* (Glu-Asp, Ala-Glu, Met-Glu, Met-Ala, Ala-Asp) in *C. utilis* extracts.
    • * Identified a lack of methionine-aspartate transamination*.
    • * Found that ethionine-resistant mutants (mec mutants) do not utilize methionine*, suggesting a transport defect.

    Conclusions:

    • * *C. utilis* possesses diverse transamination capabilities*
    • * Methionine transport is essential* for its utilization.
    • * Mec mutants exhibit a transport lesion* impairing methionine uptake.

    Related Experiment Videos