Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Antigenic association between kidney and thymocyte.

K Harada, T Yamamoto, M Hara

    Acta Pathologica Japonica
    |May 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Urinary podocytes in primary focal segmental glomerulosclerosis.

    Nephron·2001
    Same author

    FAT is a component of glomerular slit diaphragms.

    Kidney international·2001
    Same author

    [Juxtaglomerular cell tumor].

    Nihon rinsho. Japanese journal of clinical medicine·2000
    Same author

    Expression profile of extracellular matrix and its regulatory proteins during the process of interstitial fibrosis after anti-glomerular basement membrane antibody-induced glomerular sclerosis in Sprague-Dawley rats.

    Pathology international·1999
    Same author

    Origin of hyperplastic epithelial cells in idiopathic collapsing glomerulopathy.

    Histopathology·1999
    Same author

    Acute in vivo effects of ACTH by exo utero microinjection on differentiation, steroidogenesis and proliferation of fetal mouse adrenocytes.

    Endocrine research·1999

    Researchers identified a shared antigen, likely Thy 1 antigen, on rat thymocytes and renal glomeruli using immunofluorescence. Its location in the kidney varied depending on tissue fixation methods.

    Area of Science:

    • Immunology
    • Nephrology
    • Cell Biology

    Background:

    • Shared antigens between immune cells and kidney tissue are crucial for understanding autoimmune kidney diseases.
    • The Thy 1 antigen is a well-characterized marker on thymocytes, but its presence and role in renal tissue are less understood.

    Purpose of the Study:

    • To investigate the presence and localization of common antigens between rat renal tissue and lymphocytes, particularly thymocytes.
    • To characterize the specificity of antisera used in identifying these shared antigens.

    Main Methods:

    • Immunofluorescent technique was employed to detect antigens in rat tissues.
    • Absorption analysis was performed to confirm the specificity of anti-rat thymocyte serum (ATS) and anti-rat brain serum (ABS).
    • Kidney sections were examined both with and without acetone fixation to assess antigen distribution.

    Related Experiment Videos

    Main Results:

    • The brain-associated thymocyte antigen, presumed to be Thy 1 antigen, was detected on rat thymocytes and within renal glomeruli.
    • Antisera (ATS and ABS) recognized this antigen in glomeruli, with its distribution differing significantly between fixed (mesangium) and unfixed (diffuse) kidney sections.
    • Antisera against specific kidney antigens (anti-Fx1A, anti-GBM, anti-CIG) did not react with thymocytes or show absorption with brain homogenate, indicating distinct antigen specificities.

    Conclusions:

    • A shared antigen, likely Thy 1 antigen, is present in rat renal glomeruli and on thymocytes.
    • The localization of this antigen within the glomerulus is dependent on the fixation method used for kidney tissue.
    • This finding suggests potential immunological relevance of Thy 1 antigen in renal contexts.