Cell kinetics in the mouse small intestine during immediate postnatal life

The cell proliferation kinetics in the small intestine of newborn Balb/c mice were studied, from day 1 through to day 21 after birth. The size of the functional compartment and the proliferation compartment was determined as well as the cell production rate in the crypt using the micro-dissection technique combined with metaphase arrest method. The effect of weaning on cell proliferation was studied. The results suggest that: (1)There is continuous increase in cell proliferation and in the size of the functional and proliferative compartment over the 21 days. (2) The cell proliferation proceeds at a slower rate than in adult animals. (3) There is a sharp increase in cell production rate during the third week of postnatal life. (4) The cell proliferation was faster in conventionally weaned litters than the non-weaned group. (5) The intestinal mucosa in newborn mice is not in a steady-state conditions as in adults; cell production rate exceeds cell loss.