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Related Experiment Videos

Rat-liver fatty-acid-synthesizing complex.

P M Gillevet, K Dakshinamurti

    Bioscience Reports
    |October 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    High lipogenesis conditions reveal a high-molecular-weight acetyl-CoA carboxylase complex. Phosphorylation reduces its molecular weight, suggesting regulatory control over fatty acid synthesis.

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    Area of Science:

    • Biochemistry
    • Molecular Biology
    • Metabolic Regulation

    Background:

    • Lipogenesis, the process of fatty acid synthesis, is crucial for energy storage and cellular function.
    • Acetyl-CoA carboxylase (ACC) is a key regulatory enzyme in lipogenesis.
    • The multienzyme complex involved in fatty acid synthesis has been studied, but its precise composition and regulation under varying lipogenic conditions require further elucidation.

    Purpose of the Study:

    • To investigate the molecular state and enzymatic associations of acetyl-CoA carboxylase (ACC) under conditions favoring lipogenesis.
    • To determine the effect of lipogenic conditions on the levels of key lipogenic enzymes.
    • To examine the impact of phosphorylation on the molecular weight of ACC and its associated complex.

    Main Methods:

    Related Experiment Videos

  • Isolation of high-molecular-weight species of acetyl-CoA carboxylase (ACC).
  • Enzyme assays for ATP-citrate lyase, ACC, and fatty acid synthetase.
  • Co-sedimentation analysis to assess enzyme complex formation.
  • Phosphorylation experiments to study molecular weight shifts.

    • Main Results:

    • A high-molecular-weight ACC species was isolated under lipogenic conditions, distinct from in vitro polymerized ACC.
    • ATP-citrate lyase, ACC, and fatty acid synthetase were found to be associated in a high-molecular-weight complex.
    • Enzyme levels decreased under low-lipogenic conditions.
    • Phosphorylation of the isolated complex resulted in a shift to a lower molecular weight.

    Conclusions:

    • Lipogenesis involves a high-molecular-weight complex of key lipogenic enzymes, including ACC.
    • The assembly and potentially the activity of this complex are regulated by lipogenic conditions.
    • Phosphorylation serves as a regulatory mechanism, altering the molecular weight and likely the function of the lipogenic enzyme complex.