Functional postsynaptic alpha 2- but not alpha 1-adrenoceptors in dog saphenous vein exposed to phenoxybenzamine

Phenoxybenzamine greatly attenuated phenylephrine-induced contractions of dog saphenous vein in vitro, but had less effect on contractions induced by clonidine. The phenoxybenzamine-resistant responses to clonidine were not affected by prazosin or by corynanthine but were competitively antagonized by yohimbine (pA2 8.2). It is concluded that exposure of saphenous vein to phenoxybenzamine resulted in blockade of alpha 1-adrenoceptors to the extent that there remained a virtually homogeneous population of postsynaptic alpha 2-adrenoceptors. The effects of agents at postsynaptic alpha 2-adrenoceptors can be studied on this preparation without the complications caused by the presence of functional alpha 1-adrenoceptors.