Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Electrogenic Cl- pump in Acetabularia.

D Gradmann, J Tittor, V Goldfarb

    Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
    |December 1, 1982
    PubMed
    Summary
    This summary is machine-generated.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Impedance of the electrogenic Cl(-) pump inAcetabularia: Electrical frequency entrainements, voltage-sensitivity, and reaction kinetic interpretation.

    The Journal of membrane biology·2015
    Same author

    Interpretation of current-voltage relationships for "active" ion transport systems: II. Nonsteady-state reaction kinetic analysis of class-I mechanisms with one slow time-constant.

    The Journal of membrane biology·2015
    Same author

    [The effect of light, temperature, and external medium upon the electrical behaviour of Acetabularia crenulata].

    Planta·2014
    Same author

    Membrane potentials and ion permeabilities in flexor cells of the laminar pulvini of Phaseolus coccineus L.

    Planta·2014
    Same author

    ATPase activities in partially purified membranes of Acetabularia.

    Plant cell reports·2013
    Same author

    Aged preparations are electrophysiologically more representative of intact tissue than are freshly excised preparations.

    Planta·2013
    Same journal

    The microlandscapes of tree trunks: the effect of lichen and tree-level characteristics on arthropod communities.

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
    Same journal

    Centimetre-scale landscapes to assess the motion behaviour and cognition of gastropods and bivalves.

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
    Same journal

    Intertidal microcosms of wave-swept rocky shores: ecological and physiological insights from a uniquely stressful environment.

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
    Same journal

    Temporal and spatial variation in temperature and oxygen at the microscale: key niche axes for aquatic life.

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
    Same journal

    Natural microcosms in ecology: fulfilling the promise of model systems?

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
    Same journal

    Microbe-induced galls and plant defence: metabolite crosstalk in a co-evolutionary battle.

    Philosophical transactions of the Royal Society of London. Series B, Biological sciences·2026
    See all related articles

    Researchers identified an electrogenic chloride (Cl-) pump in Acetabularia, likely an ATPase, crucial for cellular function. This pump

    Area of Science:

    • Plant Physiology
    • Membrane Transport
    • Biochemistry

    Background:

    • The unicellular marine alga Acetabularia possesses an electrogenic chloride (Cl-) pump in its outer plasma membrane (plasmalemma).
    • This pump plays a role in transmembrane potential difference (V).

    Purpose of the Study:

    • To investigate the electrical characteristics of the electrogenic Cl- pump.
    • To elucidate the kinetic and mechanistic details of this charge-translocating enzyme.

    Main Methods:

    • Measurements of transmembrane potential difference (V) in vivo.
    • Analysis of steady-state current-voltage curves (Ip(V)) under dark and light conditions.
    • Application of two-state and three-state reaction kinetic models.

    Main Results:

    Related Experiment Videos

    • Evidence for a Cl- stimulated, vanadate-sensitive ATPase activity in membrane preparations.
    • A two-state kinetic model successfully predicted voltage-sensitive Cl- efflux.
    • Green light was found to disrupt the pump cycle, leading to a decrease in V.

    Conclusions:

    • The electrogenic Cl- pump is likely an ATPase.
    • A refined three-state model provides detailed insights into pump kinetics, stoichiometry, and voltage sensitivity.
    • The study enhances understanding of charge translocation mechanisms in biological membranes.