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Clofibrate does not alter cyclic nucleotide metabolism in muscle
Metabolism: Clinical and Experimental
|February 1, 1983
Summary
Clofibrate causes muscle protein breakdown, but this study found it does not involve cyclic nucleotides. Researchers found no changes in muscle cyclic nucleotide levels or related enzyme activity after clofibrate treatment.
Area of Science:
- Biochemistry
- Pharmacology
- Muscle Physiology
Background:
- Clofibrate, a hypolipidemic agent, induces muscle protein breakdown in rats and muscular syndrome in humans.
- Muscle protein metabolism is influenced by cyclic nucleotides.
- Clofibrate inhibits adenylate cyclase in adipose tissue.
Purpose of the Study:
- To investigate the role of cyclic nucleotides in clofibrate-induced muscle protein degradation.
- To determine if clofibrate affects cyclic nucleotide metabolism in muscle tissue.
Main Methods:
- Measurement of cyclic nucleotide levels (e.g., cAMP, cGMP) in muscle tissue.
- Assay of adenylate cyclase activity (basal and hormone-stimulated).
- Assessment of cyclic nucleotide phosphodiesterase activity in muscle.
Main Results:
- Clofibrate treatment did not alter cyclic nucleotide concentrations in muscle.
- No significant changes were observed in basal or hormone-stimulated adenylate cyclase activity.
- Cyclic nucleotide phosphodiesterase activity remained unaffected by clofibrate.
Conclusions:
- The mechanism of clofibrate-induced muscle protein breakdown in rats does not appear to be regulated by cyclic nucleotides.
- These findings suggest an alternative pathway for clofibrate's myotoxic effects.