Three cell lines from a spontaneous murine astrocytoma show variation in astrocytic differentiation

Three cell lines originally derived from a spontaneous, transplantable, murine astrocytoma have been maintained in vitro. Immunofluorescence confirmed their astrocytic nature while electron microscopy revealed differences in the number and ratio of 10 nm filaments and 24 nm microtubules in the 3 lines; a feature related to the degree of astrocytic differentiation.