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Related Experiment Videos

Genetic basis for Ly-6- defect: complementation between Ly-6- and Thy-1- mutant cell lines.

M A Horton, R Hyman

    Immunogenetics
    |January 1, 1983
    PubMed
    Summary

    Researchers identified cell lines with defects in multiple cell surface antigens after mutagenesis. Selecting against one antigen caused the loss of all three, indicating a pleiotropic defect. Genetic analysis revealed complementation groups for these antigen-deficient cell lines.

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    Area of Science:

    • Immunology
    • Cell Biology
    • Genetics

    Background:

    • Cell surface antigens play crucial roles in cellular recognition and function.
    • Understanding the genetic regulation of antigen expression is vital for immunology research.
    • The BW5147 cell line is a common model for studying T-cell differentiation and antigen expression.

    Purpose of the Study:

    • To investigate the genetic basis of pleiotropic cell surface antigen expression defects.
    • To analyze antigen loss variants derived from chemically mutagenized BW5147 cells.
    • To characterize the complementation groups of mutants exhibiting defects in Thy-1, Ly-6.2, and H9/25 antigens.

    Main Methods:

    • Chemical mutagenesis of the BW5147 cell line.
    • Selection of antigen loss variants using specific antibodies (anti-Thy-1.1, anti-Ly-6.2, anti-H9/25).

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  • Somatic cell hybridization for gene complementation analysis.

    • Main Results:

    • Selection for loss of any single antigen (Thy-1.1, Ly-6.2, or H9/25) resulted in the simultaneous loss of all three.
    • This indicates a pleiotropic defect affecting the expression of multiple cell surface antigens.
    • Gene complementation studies classified the Ly-6- and H9/25- mutants into different Thy-1 complementation classes (A or E).

    Conclusions:

    • A pleiotropic defect in cell surface antigen expression was identified in the selected BW5147 variants.
    • The genetic basis of this defect involves genes that regulate the expression of multiple antigens.
    • The mutants fall into distinct complementation groups, providing insights into the genetic control of antigen expression.