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Functional Complementation Analysis (FCA): A Laboratory Exercise Designed and Implemented to Supplement the Teaching of Biochemical Pathways
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Published on: June 24, 2016

Genetic basis for Ly-6- defect: complementation between Ly-6- and Thy-1- mutant cell lines

M A Horton, R Hyman

    Immunogenetics
    |January 1, 1983
    PubMed

    Selection of antigen loss variants from the BW5147 cell line after chemical mutagenesis, using antibodies to Thy-1.1, Ly-6.2 and H9/25 allospecificities, produced cell lines with a pleiotropic defect in cell surface antigen expression; selection against any antigen led to the loss of all three determinants. The genetic basis for the mutant phenotype has been analyzed by gene complementation by somatic cell hybridization between Ly-6- or H9/25- and Thy-1- mutants, which comprise different Thy-1 gene complementation classes. The Ly-6- and H9/25- mutants can be classified as belonging to either the A or E Thy-1- mutant class.

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