Related Experiment Video
Updated: Jan 25, 2026

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry
Published on: March 24, 2023
Fluorometric determination of some butyrophenones after permanganate treatment
Abstract:
A fluorometric method is described for direct and sensitive determinations of azaperone, haloperidol and bromoperidol in pharmaceutical preparations. These compounds can be converted into strongly fluorescing derivatives by the action of potassium permanganate on their alcoholic solutions in acid medium, the limits of detection being respectively 8.10(-3), 5.10(-2) microgram/ml. The fluorescence phenomenon is very stable. Preliminary results are reported on the isolation and structure determination of one of the main fluorophors, produced in the case of azaperone.
More Related Videos
07:29Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
12:33High-throughput Fluorometric Measurement of Potential Soil Extracellular Enzyme Activities
Published on: November 15, 2013
Related Concept Videos
Determination
Oxidation of Alkenes: Syn Dihydroxylation with Potassium Permanganate
Determining Order of Reaction
Determining the pH of Salt Solutions
Rate-Determining Steps
In a multistep reaction mechanism, one of the elementary steps progresses significantly slower than the others. This slowest step is called the rate-limiting step (or rate-determining step). A reaction cannot proceed faster than its slowest step, and hence, the rate-determining step limits the overall reaction rate.
The concept of rate-determining step can be understood from the analogy of a 4-lane freeway with a short-stretch of traffic-bottleneck caused due to...
Parkinson's Disease: Treatment
Parkinson's Disease is primarily a result of the loss of dopaminergic neurons in the substantia nigra pars compacta. The cornerstone of...