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Related Experiment Videos

Association between coated vesicles and microtubules.

B A Imhof, U Marti, K Boller

    Experimental Cell Research
    |April 15, 1983
    PubMed
    Summary
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    This study reveals a direct functional link between microtubules and coated vesicles. Researchers found coated vesicles attached to microtubules, suggesting a cytoskeletal role in vesicle transport and cellular organization.

    Area of Science:

    • Cell Biology
    • Cytoskeleton Dynamics
    • Vesicular Transport

    Background:

    • Microtubules are key cytoskeletal components involved in intracellular transport.
    • Coated vesicles, particularly those with clathrin, play crucial roles in protein sorting and membrane trafficking.

    Purpose of the Study:

    • To investigate a potential functional association between microtubules and coated vesicles.
    • To identify the molecular components and structural basis of this interaction.

    Main Methods:

    • Immunological methods using anti-coat antibodies to detect clathrin heavy and light chains.
    • Electron microscopy (negative staining and thin sections) to visualize structural interactions.
    • Analysis of both purified microtubule preparations and tissue culture cells.

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    Main Results:

    • Microtubule preparations contained significant amounts (over 10%) of coated vesicles.
    • Coated vesicles were predominantly attached along microtubule lengths.
    • Projections linking microtubules to the clathrin lattice of vesicles were identified, suggesting a direct interaction.
    • Similar bridges were observed between microtubules and coated vesicles in tissue culture cells.

    Conclusions:

    • A direct physical and functional association exists between microtubules and coated vesicles.
    • Microtubules likely serve as tracks or anchors for coated vesicles via specific linker structures.
    • This interaction is relevant to intracellular transport and cellular organization.