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Clofibrate-induced myotonia in rats.
Acta Neurologica Scandinavica
|April 1, 1983
Summary
Clofibrate alters rat muscle membranes, decreasing ATPase activity and changing protein profiles. These biochemical changes in clofibrate-induced myotonia were less severe than in other models.
Area of Science:
- Biochemistry
- Cell Biology
- Pharmacology
Background:
- Muscle plasma membranes and sarcoplasmic reticulum are crucial for muscle function.
- Drug-induced myotonia models are used to study muscle disorders.
- Clofibrate is a known lipid-lowering agent with potential cellular effects.
Purpose of the Study:
- To investigate the biochemical effects of clofibrate on rat muscle membranes.
- To compare the effects of clofibrate with other myotonia models.
Main Methods:
- Rats were injected with clofibrate (0.4 g/kg).
- Enzymatic activity (Na+/K+-ATPase, Mg2+-ATPase, 5'-nucleotidase) was measured.
- Protein profiles and phospholipid composition were analyzed.
Main Results:
- Plasma membranes showed decreased Na+/K+-ATPase and 5'-nucleotidase activity, with altered phosphoethanolamine levels and protein profiles.
- Sarcoplasmic reticulum exhibited reduced Mg2+-ATPase activity and protein abnormalities.
- Myofibrillar proteins shifted, with increased alpha-actinin and troponin.
Conclusions:
- Clofibrate induces biochemical alterations in rat muscle membranes, affecting enzyme activities and protein composition.
- The observed changes were less pronounced than in the 20,25-diazacholesterol model of myotonia.