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Related Experiment Videos

Increased phosphate efflux from acinar cell during protein secretion.

T Melese, S S Rothman

    The American Journal of Physiology
    |July 1, 1983
    PubMed
    Summary

    Cholinergic stimulation increases pancreatic secretion of phosphate ions, similar to sucrose and inulin. This phosphate secretion originates from cells, not paracellular transport, suggesting it doesn't use pancreatic epithelial shunts.

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    Area of Science:

    • Physiology
    • Gastroenterology
    • Cell Biology

    Background:

    • Cholinergic agonists increase pancreatic epithelium permeability to water-soluble molecules like sucrose and inulin.
    • This increased permeability is often attributed to enhanced passive paracellular shunts.
    • The role of paracellular shunts in ion transport across the pancreatic epithelium remains unclear.

    Purpose of the Study:

    • To investigate the transport mechanism of phosphate ions across the pancreatic epithelium.
    • To determine if cholinergic stimulation affects phosphate ion permeability via paracellular shunts.
    • To differentiate between cellular efflux and paracellular transport of phosphate ions in pancreatic secretion.

    Main Methods:

    • Utilized short-term organ culture of whole-rabbit pancreas with a cannulated duct.
    • Measured phosphate ion concentration in pancreatic secretions.
    • Assessed phosphate secretion rates with and without cholinergic stimulation and in varying medium phosphate concentrations.

    Main Results:

    • Cholinergic stimulation increased phosphate ion concentration in pancreatic secretions, mirroring effects on sucrose and inulin.
    • Phosphate secretion rates, both basal and stimulated, were independent of external phosphate ion concentration.
    • These findings indicate phosphate secretion reflects cellular efflux rather than paracellular transport.

    Conclusions:

    • Phosphate ions are likely excluded from paracellular shunts in the pancreatic epithelium.
    • Paracellular shunts do not significantly contribute to the transpancreatic passage of phosphate ions.
    • The pancreatic epithelium regulates phosphate ion transport primarily through cellular mechanisms.

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