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Related Experiment Videos

A rapid bioluminescence method for quantifying bacterial adhesion to polystyrene.

M J Harber, R Mackenzie, A W Asscher

    Journal of General Microbiology
    |March 1, 1983
    PubMed
    Summary
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    Bacterial adhesion of Escherichia coli to surfaces is influenced by fimbriae and flagella. Strong adhesion correlates with these structures and is inhibited by D-mannose.

    Area of Science:

    • Microbiology
    • Biochemistry

    Background:

    • Bacterial adhesion is crucial for infections.
    • Understanding Escherichia coli adhesion mechanisms is important for urinary tract infections.

    Purpose of the Study:

    • To investigate the factors influencing Escherichia coli adhesion to polystyrene surfaces.
    • To correlate adhesion with bacterial surface structures and growth phase.

    Main Methods:

    • Bioluminescence ATP analysis was used to quantify bacterial adhesion.
    • Assays were conducted at 37°C and pH 6.8 with a 10-minute incubation.
    • Presence of type 1 fimbriae and flagella were assessed.

    Main Results:

    • A >200-fold variation in adhesion capacity was observed among 34 urinary isolates of E. coli.

    Related Experiment Videos

  • Strongly adherent strains possessed both type 1 fimbriae and flagella, with maximum adhesion during the exponential growth phase.
  • D-mannose significantly inhibited attachment, while deficiency in fimbriae or flagella resulted in weak adhesion.

    • Conclusions:

    • Type 1 fimbriae and flagella are critical for strong E. coli adhesion to polystyrene.
    • Adhesion is growth-phase dependent and mediated by mannose-sensitive interactions.
    • Adherence to polystyrene correlates with uromucoid binding, not buccal or uroepithelial cells.