[Direct formation of defined compounds of benzodiazepines on thin layer]

In this work is pointed to the usage of reaction chromatography, i. e. the direct formation of defined compounds of benzodiazepine on the chromatographic plate. The benzodiazepines may be converted to benzophenone derivatives by treatment with concentr. hydrochloric acid. In cases of intoxication the reaction chromatography shortens the time of analysis and it has a greater diagnostic value. For some compounds, however, the micropreparation procedure on the chromatographic plate is preferable. The latter method is suitable for nitroderivatives which are transformed to 7-amino-derivates, occurring also as metabolites. Micropreparation involves the reduction of nitroderivatives by TiCl3.