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Methadone radioimmunoassay: two simple methods.

K Robinson, R N Smith

The Journal of Pharmacy and Pharmacology

|September 1, 1983

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Two simple and economical radioimmunoassays for methadone detection in blood and urine were developed. These assays are unaffected by common sample interferences, offering reliable methadone quantification.

Area of Science:

Clinical Chemistry
Analytical Chemistry
Pharmacology

Background:

Accurate quantification of methadone is crucial for clinical monitoring and forensic toxicology.
Existing radioimmunoassays may be affected by sample preparation or exhibit cross-reactivity with related compounds.

Purpose of the Study:

To develop and describe two simple, economical radioimmunoassays for methadone detection.
To evaluate the robustness of these assays against common sample interferences and cross-reactivity.

Main Methods:

Development of two radioimmunoassay (RIA) methods for methadone quantification.
Assay 1 utilized [1-3H](-)-methadone hydrobromide as the radiolabel.
Assay 2 employed a radioiodinated conjugate of 4-dimethylamino-2,2-diphenylpentanoic acid and L-tyrosine methyl ester.

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Main Results:

Both assays demonstrated robustness against haemolysis, decomposition, common anticoagulants, and sodium fluoride.
Negligible cross-reactivity was observed with methadone metabolites and other tested drugs.
Established cut-off levels were 30-33 ng/ml for blood and 21-24 ng/ml for urine.

Conclusions:

The developed radioimmunoassays provide simple, economical, and reliable methods for methadone quantification in biological samples.
The assays exhibit good specificity and are unaffected by common sample handling variables.
The radioiodinated conjugate assay can be further enhanced for increased sensitivity if needed.