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Updated: Aug 18, 2026

Quantification of Fungal Colonization, Sporogenesis, and Production of Mycotoxins Using Kernel Bioassays
Published on: April 23, 2012
Incidence and control of mycotoxin producing molds in domestic and imported cheeses
Abstract:
Molds were isolated from moldy cheese trimmings and both nonmoldy and moldy samples of domestic and imported retail samples of cheese. The majority of molds found were Penicillium species. A small percentage (about 4 p. 100) of the molds encountered were species known to be capable of producing recognized mycotoxins (P. cyclopium, P. viridicatum, A. flavus and A. ochraceus). Most of the molds isolated has psychrotrophic properties and were capable of growing at 5 degrees C. Sorbates were found to be partially effective in delaying the growth of molds obtained from cheese. However, 65--75 p. 100 of the mold isolates were capable of initiating growth in the presence of 0.3 p. 100 potassium sorbate within 7 days at 5 degrees C. Pimaricin, on the other hand, prevented the growth of all the mold isolates at 12 and 5 degrees C and of 96 p. 100 of the isolates at 25 degrees C for at least 7 days.
Insights
This study identified Penicillium molds in cheese, with some producing mycotoxins. Pimaricin effectively inhibited mold growth, while potassium sorbate showed limited effectiveness in preserving cheese quality.
Area of Science:
- Food microbiology
- Mycology
- Food safety
Background:
- Molds are common contaminants in cheese, posing potential health risks due to mycotoxin production.
- Certain mold species found in cheese are known to produce harmful mycotoxins.
- The psychrotrophic nature of many mold isolates necessitates effective preservation strategies.
Purpose of the Study:
- To identify mold species present in various cheese samples.
- To assess the mycotoxin-producing potential of isolated molds.
- To evaluate the efficacy of potassium sorbate and pimaricin as mold inhibitors in cheese.
Main Methods:
- Isolation and identification of mold species from domestic and imported cheese samples.
- Testing mold growth at different temperatures (5, 12, and 25 degrees C).
- Assessing the inhibitory effects of potassium sorbate and pimaricin on mold growth.
Main Results:
- Penicillium species were the predominant molds isolated from cheese.
- A small proportion of isolates (approx. 4%) belonged to mycotoxin-producing species.
- Potassium sorbate demonstrated partial effectiveness, with 65-75% of isolates growing in its presence.
- Pimaricin inhibited the growth of nearly all mold isolates across tested temperatures.
Conclusions:
- Pimaricin is a highly effective agent for preventing mold growth on cheese.
- Potassium sorbate offers limited protection against mold contamination in cheese under the tested conditions.
- Effective mold control strategies are crucial for ensuring cheese safety and shelf-life.
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