Immunoreactive dynorphin and alpha-neo-endorphin in rat hypothalamo-neurohypophyseal system

Concentrations of dynorphin and alpha-neo-endorphin were measured in rat supraoptic and paraventricular nuclei, median eminence and posterior pituitary. Paraventricular lesions did not alter the level of either peptide in the median eminence or posterior pituitary. Knife cuts in the lateral retrochiasmatic area that severed the supraoptico-hypophyseal tract reduced dynorphin and alpha-neo-endorphin levels by 64-80% in the posterior pituitary and by somewhat less in the median eminence. Knife cuts in the vicinity of the supraoptic nucleus that did not interrupt the supraoptico-hypophyseal tract did not affect peptide concentrations in the posterior pituitary. Our data suggest that dynorphin and alpha-neo-endorphin in the posterior pituitary are in processes of supraoptic nucleus neurons. These neurons also project to the median eminence which may receive fibers from other dynorphin and alpha-neo-endorphin containing cells too.