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A new sensitive, thiol tolerant method for the determination of inorganic pyrophosphatase
Acta Chemica Scandinavica. Series B: Organic Chemistry and Biochemistry
|January 1, 1983
Abstract:
In this work a new sensitive, thiol tolerant method for the determination of inorganic pyrophosphatase (EC 3.6.1.1) was developed. It is based on the formation of phosphomolybdate-triethylamine complex, which in acid-acetone mixture forms a white, homogeneous precipitate, the turbidity of which can accurately measured spectrophotometrically at 355 nm. This method can be easily applied to all the Pi-producing enzymes whose substrates are stable enough in our acidic experimental conditions.