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The competition between adenosine triphosphate and inorganic pyrophosphate for myosin and its suppression by
Biochimica Et Biophysica Acta
|December 28, 1983
Abstract:
Inorganic pyrophosphate (PPi) inhibits not only Mg2+-ATPase activity of myosin subfragment 1 (S-1) but abolishes also the ATP-induced increment of tryptophan fluorescence of subfragment 1. At the concentrations used (25-50 micron ATP, 12 mm PPi) these effects of PPi were abolished by substoichiometric actin concentrations (approx. 0.1 microM actin vs. approx. 1 microM S-1), where ATPase activity was barely stimulated by actin.