Related Experiment Videos
Behavioral analysis of benzodiazepine-mediated inhibition in the early chick embryo
Diazepam, a depressant benzodiazepine, produced a dose-dependent decrease in the spontaneous motility of 5-day embryos while Ro 5-3663, a convulsant benzodiazepine, had no apparent effect. Diazepam and 4 other benzodiazepines inhibited motility in 5-day embryos with a potency that paralleled their effectiveness in ligand-binding studies. Ro 11-5073/Ro 11-5231, depressant benzodiazepine enantiomers, stereospecifically inhibited motility in 4- and 5-day embryos. Ro 15-1788, a benzodiazepine antagonist, reversed the decrease in the motility of 4- and 5-day embryos caused by a depressant benzodiazepine, clonazepam. Ro 5-6945, a potent agonist for the non-neuronal (but not the neuronal) benzodiazepine receptor, had no apparent effect on motility in 5-day embryos. Benzodiazepine receptors appear at least as early as day 5 (and perhaps as early as day 4) in the chick embryo.
Diazepam, a depressant benzodiazepine, produced a dose-dependent decrease in the spontaneous motility of 5-day embryos while Ro 5-3663, a convulsant benzodiazepine, had no apparent effect. Diazepam and 4 other benzodiazepines inhibited motility in 5-day embryos with a potency that paralleled their effectiveness in ligand-binding studies. Ro 11-5073/Ro 11-5231, depressant benzodiazepine enantiomers, stereospecifically inhibited motility in 4- and 5-day embryos. Ro 15-1788, a benzodiazepine antagonist, reversed the decrease in the motility of 4- and 5-day embryos caused by a depressant benzodiazepine, clonazepam. Ro 5-6945, a potent agonist for the non-neuronal (but not the neuronal) benzodiazepine receptor, had no apparent effect on motility in 5-day embryos. Benzodiazepine receptors appear at least as early as day 5 (and perhaps as early as day 4) in the chick embryo.