Extrinsic control of phasic supraoptic neurones in vitro: burst initiation and termination following brief changes in...

Experiments were performed to investigate the responses of phasic supraoptic cells in hypothalamic slices to brief (2-3 sec) changes in excitatory drive, induced by varying the level of local glutamate stimulation. A brief increase in excitation during a silent period immediately triggered a prolonged burst of firing, similar in duration to the ongoing phasic bursts. A transient decrease in excitation during a phasic burst evoked a protracted quiescence, reminiscent of a typical silent period. These results support the idea that in phasic cells the effects of brief synaptic inputs may be amplified in time to produce a prolonged change in electrical activity and associated hormone release.