Isolation and partial sequence of bovine cDNA clones for the high-mobility-group protein (HMG-1)

Several cloned ds cDNAs containing bovine HMG-1 sequences have been isolated from a ds cDNA library prepared from the poly(A)+ mRNA fraction of bovine testis using a pool of synthetic 17-meric oligo-deoxyribonucleotides with the sequence 5'-GTAGTTAGTTCCACATTCTC-3' selected to be complementary to a region of the coding sequence corresponding to the relatively unambiguous amino acid sequence, Glu-Met-Trp-Asn-Asn-Thr. Determination of the DNA sequences in these clones indicates that they represent the 3' half of the HMG-1 message and contain an unusually long putative 3' untranslated region of 480 nucleotides. The sequence of the coding region corresponding to the 99 amino acids at the C-terminus of HMG-1 has been determined and largely confirms the published primary sequence in this region (Walker JM, (1982) in: The HMG Chromosomal Proteins, Academic Press, London & New York, pp. 69-88). In addition the cDNA sequence provides a complete sequence of the 30 residue polyacidic region and shows that the nucleotide sequence in this region is a repeating one and that the polyacidic domain comprises the C-terminus of the protein.