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Related Experiment Videos

Cellular distribution of thyroid myosin.

M Tawata, Y Totsuka, T Nielsen

    Endocrinology
    |March 1, 1984
    PubMed
    Summary
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    Thyroid cells contain nonmuscle myosin associated with plasma membranes, not smooth muscle myosin. This myosin

    Area of Science:

    • Cell Biology
    • Molecular Biology
    • Endocrinology

    Background:

    • Myosin, a motor protein, plays crucial roles in cellular functions.
    • Thyroid hormone secretion involves endocytosis and exocytosis.

    Purpose of the Study:

    • To investigate the subcellular localization and characteristics of myosin in thyroid cells.
    • To determine the association of myosin with thyroid plasma membranes.

    Main Methods:

    • Immunofluorescence microscopy using antisera to nonmuscle and smooth muscle myosins.
    • Biochemical analysis of isolated bovine thyroid plasma membranes and cytoskeletal fractions.
    • Sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and myosin light chain kinase phosphorylation assays.

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    Main Results:

    • Thyroid epithelial cells possess nonmuscle myosin, localized at the cell periphery and intracellularly.
    • Myosin heavy and light chains were identified in the thyroid cytoskeletal fraction.
    • Myosin exhibits calcium- and calmodulin-dependent phosphorylation and EDTA-K+ ATPase activity.
    • Myosin is associated with thyroid plasma membranes, likely via actin filaments.

    Conclusions:

    • Nonmuscle myosin is present in thyroid epithelial cells and associated with the plasma membrane.
    • This myosin association may be critical for endocytic and exocytic processes in thyroid hormone secretion.