Establishment of a mosquito cell line from Haemagogus equinus larvae

The establishment and characterization of a Haemagogus equinus mosquito cell line (GML-HE-12) are described. The cells are diploid (2N = 6) and seem to be free of contaminants. Their susceptibility to 13 arboviruses was tested. Eleven of the viruses multiplied in this cell line; six of these viruses still showed titers of 4 log10 plaque forming unit/ml or greater at 33 d postinoculation. No overt cytopathologic effect was observed.