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Related Experiment Videos

Mouse paw anaphylaxis.

S Kaneta, S Ishizuki, E Fujihira

    Journal of Immunological Methods
    |June 8, 1984
    PubMed
    Summary
    This summary is machine-generated.

    This study developed a mouse paw anaphylaxis model to assess homocytotropic antibodies. The model proved effective for screening anti-allergic drugs, demonstrating significant protection with certain antihistamines.

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    Area of Science:

    • Immunology
    • Pharmacology

    Background:

    • Anaphylaxis is a severe allergic reaction.
    • Homocytotropic antibodies play a key role in anaphylaxis.

    Purpose of the Study:

    • To investigate anaphylactic swelling passively transferred to mouse paws.
    • To establish a reliable method for assessing homocytotropic antibodies.
    • To evaluate the efficacy of various drugs in preventing anaphylaxis.

    Main Methods:

    • Passive transfer of homologous antibodies (IgG1) to mouse paws.
    • Induction of anaphylaxis via antigen challenge.
    • Measurement of paw edema using a thickness gauge.
    • Histological examination of mast cell degranulation.
    • Screening of anti-allergic drugs for protective effects.

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    Main Results:

    • Maximal paw edema occurred 15 minutes after antigen challenge.
    • Heating antibodies reduced activity by 40%.
    • Paw anaphylaxis involved mast cell degranulation and mediator release.
    • Several antihistamines (promethazine, pyrilamine, diphenhydramine, etc.) provided significant protection.
    • Bromocriptine, dexamethasone, and indomethacin were ineffective.

    Conclusions:

    • Mouse paw anaphylaxis is a convenient model for quantifying homocytotropic antibodies.
    • This model is useful for routine screening of potential anti-allergic drugs.
    • The findings contribute to understanding anaphylaxis mechanisms and drug development.