Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Videos

Tumor cells adhere to intraperitoneal injection sites.

R Kühnlein, E A Friedrich, V Burkart

    Virchows Archiv. B, Cell Pathology Including Molecular Pathology
    |January 1, 1984
    PubMed
    Summary

    Tumor cells rapidly form plaques after intraperitoneal injection, indicating passive transport. Dihydrocortisol and silica particles disrupt this process by affecting cell adherence and macrophages.

    Related Concept Videos

    You might also read

    Related Articles

    Articles linked to this work by shared authors, journal, and citation graph.

    Sort by
    Same author

    Meal-derived glucagon responses are related to lower hepatic phosphate concentrations in obesity and type 2 diabetes.

    Diabetes & metabolism·2018
    Same author

    Inverse association of insulin antibody levels with insulin sensitivity in adults with Type 1 diabetes.

    Diabetic medicine : a journal of the British Diabetic Association·2018
    Same author

    Fatty acids modulate cytokine and chemokine secretion of stimulated human whole blood cultures in diabetes.

    Clinical and experimental immunology·2013
    Same author

    Differently complex oligosaccharides can be easily identified by matrix-assisted laser desorption and ionization time-of-flight mass spectrometry directly from a standard thin-layer chromatography plate.

    Journal of chromatography. A·2010
    Same author

    Image-guided and passively tumour-targeted polymeric nanomedicines for radiochemotherapy.

    British journal of cancer·2009
    Same author

    Heat shock protein 60: regulatory role on innate immune cells.

    Cellular and molecular life sciences : CMLS·2007

    Area of Science:

    • Oncology
    • Immunology
    • Cell Biology

    Background:

    • Intraperitoneal tumor cell injection is a common method to study cancer metastasis.
    • The early mechanisms of tumor cell adhesion and plaque formation are not fully understood.

    Purpose of the Study:

    • To investigate the rapid formation of tumor cell plaques following intraperitoneal injection in mice.
    • To elucidate the role of passive transport, chemotaxis, and cellular components in this process.

    Main Methods:

    • Intraperitoneal injection of tumor cells into mice.
    • Administration of dihydrocortisol to block cell adherence.
    • Use of silica particles to investigate macrophage involvement.

    Main Results:

    • Tumor cells formed plaques at injection sites within minutes, suggesting passive transport.
    • Chemotactic factors were not implicated in the initial cell plaque formation.
    • Dihydrocortisol inhibited tumor cell adherence, and silica particles abolished plaque formation, implicating macrophages.

    Conclusions:

    • Early tumor cell aggregation after intraperitoneal injection is primarily a passive process.
    • Cell adherence mechanisms and macrophage activity are critical for initial tumor cell plaque formation.

    Related Experiment Videos