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Related Experiment Videos

Immunosuppression in new-born lambs.

J Broekman, P A Eksteen, D W Verwoerd

    The Onderstepoort Journal of Veterinary Research
    |September 1, 1977
    PubMed
    Summary

    A new cytotoxicity-neutralization assay effectively titrates anti-lymphocyte and anti-macrophage sera. High-titre sera demonstrated in vivo immunosuppressive activity in lambs, confirming assay validity.

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    The effect of a natural maedi-visna virus infection on the productivity of South African sheep.

    The Onderstepoort journal of veterinary research·2000

    Area of Science:

    • Immunology
    • In vitro assays
    • Animal models

    Background:

    • Accurate titration of antisera is crucial for understanding immune responses.
    • Existing methods like agglutination assays may not fully capture functional antibody activity.

    Purpose of the Study:

    • To develop and validate a novel in vitro cytotoxicity-neutralization assay.
    • To compare the novel assay's results with a traditional agglutination assay.
    • To assess the in vivo immunosuppressive activity of anti-lymphocyte sera identified by the novel assay.

    Main Methods:

    • Developed a cytotoxicity-neutralization assay for anti-sheep lymphocyte and anti-sheep macrophage sera.
    • Titered several antisera using both the novel assay and a standard agglutination assay.
    • Administered high-titre anti-lymphocyte sera to newborn lambs and monitored peripheral blood lymphocyte counts.

    Main Results:

    • The cytotoxicity-neutralization assay provided reliable titration of antisera.
    • A strong correlation between high cytotoxicity-neutralization titres and effective in vivo suppression of circulating lymphocytes was observed.
    • Anti-lymphocyte sera with high titres in the novel assay demonstrated significant immunosuppressive effects in treated lambs.

    Conclusions:

    • The cytotoxicity-neutralization assay is a valid and effective method for titrating lymphocyte and macrophage antisera.
    • This assay can predict in vivo immunosuppressive activity.
    • The findings support the utility of this novel assay in immunological research and diagnostics.

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