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Related Experiment Videos

Morphometry of platelet internal contraction.

J G White, S M Burris

    The American Journal of Pathology
    |June 1, 1984
    PubMed
    Summary
    This summary is machine-generated.

    Platelet shape change involves microtubule reorganization. Microtubule ring diameter significantly decreases upon activation, supporting the idea that platelet internal transformation is a contractile process.

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    Redistribution of GPIb/IX and GPIIb/IIIa during spreading of discoid platelets.

    British journal of haematology·1995

    Area of Science:

    • Cell Biology
    • Hematology

    Background:

    • Blood platelets possess a discoid shape maintained by microtubules.
    • Platelet activation leads to shape change, pseudopod extension, and internal reorganization.
    • The contractile nature of platelet internal transformation remains debated due to microtubule dynamics.

    Purpose of the Study:

    • To investigate the role of microtubules in platelet internal transformation.
    • To determine if microtubule dynamics are essential for platelet shape change and internal reorganization.

    Main Methods:

    • Measurement of microtubule coil diameters in resting and activated platelets.
    • Assessment of Taxol's effect on microtubule structure and platelet function.
    • Platelet activation using thrombin, ADP, and a foreign surface.

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    Main Results:

    • No significant difference in microtubule ring diameter was observed between control and Taxol-treated platelets.
    • Platelet activation by ADP, thrombin, or a foreign surface induced a ≥30% decrease in microtubule coil diameter.
    • Taxol did not inhibit platelet shape change, internal transformation, secretion, aggregation, or clot retraction.

    Conclusions:

    • Platelet internal transformation is a contractile event.
    • Microtubule dynamics, specifically the decrease in coil diameter, are crucial for platelet shape change and internal reorganization.