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Related Experiment Videos

Methylamine and islet function: possible relationship to Ca2+-sensitive transglutaminase.

A Sener, R Gomis, P Lebrun

    Molecular and Cellular Endocrinology
    |July 1, 1984
    PubMed
    Summary

    Methylamines inhibit insulin release from pancreatic islet cells. This effect is not always linked to the inhibition of transglutaminase activity, with calcium concentration playing a key role.

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    Area of Science:

    • Biochemistry
    • Endocrinology
    • Cell Biology

    Background:

    • Pancreatic islet cells are crucial for glucose homeostasis.
    • Transglutaminase activity in islets is calcium-dependent.
    • Methylamine compounds are known to affect cellular processes.

    Purpose of the Study:

    • To investigate the relationship between transglutaminase activity and insulin release in pancreatic islets.
    • To determine the role of calcium concentration in methylamine-induced inhibition of insulin secretion.

    Main Methods:

    • Preparation of pancreatic islet homogenates.
    • Assay of transglutaminase activity in the presence of varying methylamine concentrations and calcium levels.
    • Measurement of insulin release from intact islet cells under different conditions.

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  • Incorporation studies of methylamine into islet proteins.

    • Main Results:

    • Pancreatic islet homogenates exhibit calcium-dependent transglutaminase activity.
    • Methylamine inhibits both transglutaminase activity and glucose-stimulated insulin release.
    • The inhibitory effects of methylamine on enzyme and insulin release are inversely related to calcium concentration.
    • Dimethylamine also inhibits transglutaminase and insulin release, while trimethylamine inhibits insulin release without affecting transglutaminase activity.

    Conclusions:

    • The inhibition of insulin release by methylamines is not solely dependent on the inhibition of transglutaminase activity.
    • Calcium concentration modulates the effects of methylamines on both transglutaminase activity and insulin secretion.
    • These findings suggest complex mechanisms underlying methylamine-induced alterations in pancreatic islet function.