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Thy-1.1 in glomeruli of rat kidneys
Kidney International
|May 1, 1984
Summary
The Thy-1.1 antigen is present on rat kidney glomerular mesangial cells. This finding was determined using immunofluorescence and immunoelectron microscopy with specific monoclonal antibodies.
Area of Science:
- Immunology
- Nephrology
- Cell Biology
Background:
- The rat kidney's antigenic composition is not fully characterized.
- Understanding kidney antigens is crucial for diagnosing and treating renal diseases.
Purpose of the Study:
- To investigate the expression and localization of the Thy-1.1 antigen in the rat kidney.
- To identify specific kidney cell types expressing Thy-1.1.
Main Methods:
- Indirect immunofluorescence microscopy.
- Immunoelectron microscopy.
- Monoclonal antibodies against Thy-1.1 (OX7, ER Ly 1), Thy-1.2 (30-H12), and W3/13.
- Rabbit antisera against rat brain or thymic blasts.
- Blocking and absorption experiments.
- In vivo perfusion studies.
Main Results:
- Anti-Thy-1.1 reagents strongly stained the glomerular mesangium.
- Weak staining was observed around medullary collecting ducts/vessels.
- Additional antibodies in rabbit antisera bound to renal basement membranes and smooth muscle cells.
- Immunoelectron microscopy confirmed OX7 antibody binding to mesangial cells and matrix in isolated glomeruli.
Conclusions:
- The Thy-1.1 antigen is expressed on glomerular mesangial cells in rat kidneys.
- This study identifies a specific cell surface marker for rat glomerular mesangial cells.