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Substrate utilization by Legionella cells after cryopreservation in phosphate buffer.

E Weiss, H N Westfall

    Applied and Environmental Microbiology
    |August 1, 1984
    PubMed
    Summary
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    This study found that glutamate is an effective energy source for Legionella, even after cryopreservation. Certain buffers and salts impact metabolic activity, with alpha-ketoglutarate likely not being a primary energy source.

    Area of Science:

    • Microbiology
    • Bacterial Physiology

    Background:

    • Legionella growth media require specific buffers and energy sources for optimal cultivation.
    • Understanding bacterial metabolism is crucial for effective growth and study of Legionella species.

    Purpose of the Study:

    • To assess the efficacy of common buffers and energy sources in Legionella growth media using metabolic tests.
    • To evaluate the impact of cryopreservation on bacterial metabolic activity.

    Main Methods:

    • Metabolic activity was assessed by measuring carbon dioxide production and incorporation from 14C-labeled substrates.
    • Legionella pneumophila, L. micdadei, and L. bozemanii cells were tested in various buffers with MgSO4.
    • Cryopreservation effects on metabolic activity were examined.

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    Main Results:

    • Potassium phosphate buffer was suitable for glutamate metabolism, but KCl or NaCl addition decreased activity.
    • Metabolic activity for glutamate remained stable after cryopreservation.
    • High metabolic activity was observed for serine, glutamate, threonine, and pyruvate; lower activity was seen for alpha-ketoglutarate, succinate, and gamma-aminobutyrate.
    • Glutamate preferentially spared other amino acids for synthesis when used as an energy source.

    Conclusions:

    • Glutamate is a valuable energy source for Legionella, potentially sparing other amino acids.
    • Alpha-ketoglutarate, while possibly mitigating oxygen toxicity, is unlikely to be a primary energy source for Legionella.