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Related Experiment Videos

Cryopreserved human thyroid tissue for TSAb determination: comparison with fresh tissue.

L M Tanaka-Matsuura, R S Werner, C S Farah

    Hormone Research
    |January 1, 1984
    PubMed
    Summary

    Cryopreservation of human thyroid slices maintains sensitivity for thyroid-stimulating antibody (TSAb) assays. This method is effective for TSAb determinations, enabling reliable cyclic-AMP generation measurements.

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    Area of Science:

    • Endocrinology
    • Immunology
    • Cell Biology

    Background:

    • Thyroid-stimulating antibodies (TSAbs) are key diagnostic markers for Graves' disease.
    • Assessing TSAb activity typically requires fresh human thyroid tissue, limiting its widespread use.
    • Cryopreservation offers a potential method for preserving biological samples for diagnostic assays.

    Purpose of the Study:

    • To evaluate the efficacy of a cryopreservation technique for human normal thyroid slices.
    • To determine if cryopreserved thyroid slices can serve as a sensitive substrate for cyclic-AMP generation in response to thyroid-stimulating antibodies.
    • To compare the performance of fresh versus cryopreserved thyroid slices in a thyroid-stimulating antibody assay.

    Main Methods:

    • Human normal thyroid slices were cryopreserved using a sterile 15% glycerol and 0.9% NaCl solution, with storage at -80°C for 1-5 weeks.

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  • Thyroid-stimulating antibody (TSAb) assays were conducted using both fresh and cryopreserved thyroid slices.
  • Dose-response curves for bovine thyroid-stimulating hormone (b-TSH) and Graves' hyperthyroid patient IgG were generated.
  • Cyclic-AMP generation was measured as an indicator of thyroid cell stimulation.

    • Main Results:

    • Dose-response curves for b-TSH and Graves' IgG were comparable between fresh and cryopreserved thyroid slices.
    • The assay sensitivity for TSH was 5 mU/ml.
    • A high correlation (r = 0.91; p < 0.001) was observed for cyclic-AMP increase between fresh and cryopreserved methods when stimulated by b-TSH and Graves' IgG.
    • Positive TSAb results were obtained in 63% of patients using fresh slices and 52% using cryopreserved slices.

    Conclusions:

    • The cryopreservation technique effectively preserves the functional integrity of human thyroid slices for TSAb determinations.
    • Cryopreserved thyroid slices provide a sensitive and reliable substrate for measuring cyclic-AMP generation.
    • This cryopreservation method enhances the feasibility of TSAb assays, potentially improving diagnostic accessibility for Graves' disease.