Localization of tyrosine-hydroxylase immunoreactive cells in rabbit retinal cultures

Tyrosine-hydroxylase immunoreactivity is demonstrable by the indirect immunofluorescence technique in a small sub-population of retinal neurones cultured from 2-day-old rabbits. Staining was only weak, restricted to a few neurones, and could not be observed in cultures of less than 10 days. Autoradiographical analysis of [3H]dopamine uptake by retinal cultures strongly suggests that neurones containing tyrosine-hydroxylase immunoreactivity have the capacity to take up exogenous dopamine. These findings are in concordance with studies on rabbit retinal preparations.