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The factor B and C2 genes.

R D Campbell, D R Bentley, B J Morley

    Philosophical Transactions of the Royal Society of London. Series B, Biological Sciences
    |September 6, 1984
    PubMed
    Summary
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    Researchers characterized the Factor B gene, a complement system component, finding it located near the C2 gene within the major histocompatibility complex. Gene structure analysis revealed novel exon organization, suggesting evolutionary insights into serine proteinases.

    Area of Science:

    • Immunology
    • Molecular Biology
    • Genetics

    Background:

    • Factor B and C2 are serine proteinases within the complement system.
    • These genes are located in the major histocompatibility complex.
    • Understanding their genetic structure is key to their function and evolution.

    Purpose of the Study:

    • To isolate and characterize the genomic DNA containing the Factor B and C2 genes.
    • To analyze the structure of the Factor B gene, including its exons and introns.
    • To compare the Factor B gene's exon organization with other serine proteinases to infer evolutionary relationships.

    Main Methods:

    • cDNA probes were used to isolate cosmid clones containing Factor B and C2 genes.
    • Southern blot analysis determined gene loci proximity.

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  • DNA sequence analysis characterized the Factor B gene's length, exon count, and specific sequences.

    • Main Results:

    • Single Factor B and C2 loci were identified, located less than 1 kilobase apart.
    • The Factor B gene is approximately 6 kilobases long and comprises 18 exons.
    • Homologous regions in the Ba fragment and serine proteinase domain are encoded by separate exons, with one unique exon in Factor B.

    Conclusions:

    • The genetic organization of Factor B suggests evolution through DNA duplication events.
    • The unique exon in Factor B may have functional significance.
    • Comparative analysis provides insights into the evolution of novel serine proteinases.