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Establishment of a long-term cultured suppressor T-cell line specific for guanosine
Induction of suppressor cells specific for guanosine in C57BL/6 mice with syngeneic spleen cells coupled with guanosine was demonstrated. Spleen cells from mice that had been tolerized by the injection of guanosine-coupled syngeneic spleen cells (G-SC) were repeatedly stimulated with mitomycin C-treated G-SC (MMC-G-SC) in vitro. These cells effectively suppressed the secondary in vitro response to guanosine-keyhole limpet haemocyanin (G-KLH) but did not suppress the response to sheep erythrocytes (SRBC). From these cell populations a long-term-cultured suppressor T-cell line specific for guanosine was established by using interleukin-2 and MMC-G-SC. This cell line suppressed the response to G-KLH but did not suppress the response to trinitrophenyl-KLH and SRBC. The suppressive effect was completely eliminated by treatment with anti-Thy 1.2 and complement.
Induction of suppressor cells specific for guanosine in C57BL/6 mice with syngeneic spleen cells coupled with guanosine was demonstrated. Spleen cells from mice that had been tolerized by the injection of guanosine-coupled syngeneic spleen cells (G-SC) were repeatedly stimulated with mitomycin C-treated G-SC (MMC-G-SC) in vitro. These cells effectively suppressed the secondary in vitro response to guanosine-keyhole limpet haemocyanin (G-KLH) but did not suppress the response to sheep erythrocytes (SRBC). From these cell populations a long-term-cultured suppressor T-cell line specific for guanosine was established by using interleukin-2 and MMC-G-SC. This cell line suppressed the response to G-KLH but did not suppress the response to trinitrophenyl-KLH and SRBC. The suppressive effect was completely eliminated by treatment with anti-Thy 1.2 and complement.