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Updated: Aug 12, 2026
08:58
Enhanced Rabies Surveillance Using a Direct Rapid Immunohistochemical Test
Published on: April 30, 2019
Enzyme-linked immunosorbent assay for quantification of rabies antibodies in human sera
Acta Virologica
|September 1, 1984
Abstract:
A double antibody enzyme linked immunosorbent assay (ELISA) was elaborated for detection of rabies antibodies in human sera. The procedure consisted of coating polyvinylchloride plates with rabbit antirabies serum followed by attachment of partially purified fixed virus and human rabies antibodies. The rabies-specific antibodies in human antisera were quantified by means of antihuman peroxidase conjugate. Titration of antisera from human volunteers immunized with the "Fermi" vaccine revealed excellent correlation of the virus neutralization test and ELISA.
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01:33Enzyme-Linked Immunosorbent Assay
In 1971, Peter Perlman and Eva Engvall developed an Enzyme-linked immunosorbent assay (ELISA or EIA). ELISA differs from western blot in that the assays are conducted in microtiter plates or in vivo rather than on an absorbent membrane.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
There are many different types of ELISAs, but they all involve an antibody molecule whose constant region binds an enzyme, leaving the variable region free to bind its specific antigen. Enzyme-substrate reaction allows the antigen to be visualized or quantified.
01:28Rabies
Rabies is a lethal zoonotic disease caused by a single-stranded, negative-sense RNA virus of the Lyssavirus genus, within the family Rhabdoviridae. Its primary mode of transmission to humans is through bites or saliva-contaminated scratches from infected mammals such as dogs, bats, raccoons, or foxes. Transmission can also occur if infectious saliva contacts abraded skin or intact mucous membranes, including the conjunctiva.Viral Entry and Early ReplicationOnce introduced at the bite or scratch...