Intracellular Ca2+ elevation induced by a neurotransmitter in a glial cell clone

By fluorometry using a Ca2+-indicator Quin 2, we found an elevation of intracellular Ca2+ concentration ([Ca2+]i) in response to an application of serotonin in a rat clonal glial cell (C6BU-1). The [Ca2+]i rise depended on the dose of applied serotonin and the level of environmental Ca2+. The possibility was suggested that neuron-glia interactions might be controlled by a receptor-coupled [Ca2+]i-regulation system.