Regulation of secretion from serous and mucous cells in the trachea

The physical properties of mucus and the efficiency of tracheal mucociliary clearance depend on maintenance of a balanced interaction among several epithelial cell types. Some of these cell types are specialized to perform ion and water transport, others to perform synthesis and secretion of macromolecules. Our studies have been aimed specifically at identifying the neural mechanisms regulating macromolecule secretion from two of these cell types, i.e. serous and mucous gland cells. Because these cells occur as part of a complex epithelium, it is difficult to monitor the properties and functions of each cell type individually. We have therefore relied principally on morphological methods, which can potentially focus on a single cell type within a heterogeneous tissue. Such studies, however, depend on the availability of visible markers (enzyme-labelled antibodies, radioligands, etc.), and many important aspects of gland cell function cannot be assessed morphologically. Two alternative approaches are therefore being developed: the isolation and segregation of gland cells according to type, and the production of monoclonal antibodies that recognize secretory products of individual cell types. These methods allow serous and mucous cells to be studied by biochemical as well as morphological methods.