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Interaction of basic compounds with coated vesicles.

A Di Cerbo, P K Nandi, H Edelhoch

    Biochemistry
    |December 4, 1984
    PubMed
    Summary
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    Poly- and dibasic amines, like chloroquine, inhibit coated vesicle dissociation. Aromatic ring systems in these compounds enhance stabilization of the vesicle coat structure.

    Area of Science:

    • Cell Biology
    • Biochemistry

    Background:

    • Coated vesicles are crucial for receptor-mediated endocytosis.
    • Understanding the factors that regulate coated vesicle dissociation is important for cellular processes.

    Purpose of the Study:

    • To investigate the effect of poly- and dibasic amines on coated vesicle dissociation.
    • To determine the contribution of amine groups and ring systems to the stabilization of coated vesicle structure.

    Main Methods:

    • Light scattering assays were used to monitor vesicle dissociation.
    • Sucrose gradient centrifugation was employed to analyze the effects of compounds on coated vesicles.

    Main Results:

    • Polybase effectiveness in inhibiting dissociation correlated with the number of amine groups.

    Related Experiment Videos

  • Dibasic aliphatic amines showed minimal differences in effectiveness.
  • Chloroquine and quinacrine were more effective inhibitors than dibasic aliphatic amines, suggesting a role for their aromatic ring systems.

    • Conclusions:

    • Aromatic ring systems contribute to the stabilization of coated vesicle structure.
    • Interactions between poly/dibasic compounds and clathrin/coated vesicles may influence ligand turnover in endocytosis.