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Related Experiment Videos

Ethanol and temperature effects on five membrane bound enzymes.

A C Collins, A Smolen, A L Wayman

    Alcohol (Fayetteville, N.Y.)
    |May 1, 1984
    PubMed
    Summary

    Ethanol affects membrane-bound enzymes differently in mice bred for varied sleep durations. Specifically, it impacts the low-affinity ouabain NaK-ATPase, correlating with ethanol sensitivity differences between long-sleep and short-sleep mice.

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    Area of Science:

    • Neuropharmacology
    • Biochemistry
    • Genetics

    Background:

    • Long-sleep (LS) and short-sleep (SS) mice exhibit distinct responses to ethanol, particularly in sleep duration.
    • Membrane-bound enzymes are crucial for cellular function and can be targets of ethanol's effects.
    • Understanding enzyme activity in LS and SS mice can elucidate the molecular basis of ethanol sensitivity.

    Purpose of the Study:

    • To investigate the effects of ethanol on the activity of five specific membrane-bound enzymes in LS and SS mouse cortex.
    • To determine if ethanol's impact on enzyme activity correlates with the genetic differences in ethanol sensitivity between LS and SS mice.

    Main Methods:

    • Crude membrane preparations from LS and SS mouse cortex were utilized.
    • Activities of NaK-ATPase (two forms), Mg-ATPase, 5'nucleotidase, and acetylcholinesterase were measured.

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  • Arrhenius plots were employed to analyze enzyme activity in response to varying temperatures and ethanol concentrations.

    • Main Results:

    • Ethanol specifically affected the high ouabain-sensitive NaK-ATPase, reducing its transition temperature more in LS than SS membranes.
    • Ethanol did not alter the transition temperature of the high-affinity ouabain NaK-ATPase, Mg-ATPase, 5'nucleotidase, or acetylcholinesterase.
    • Differences in control transition temperatures were observed for high-affinity NaK-ATPase and acetylcholinesterase between LS and SS mice.

    Conclusions:

    • Ethanol exhibits specificity in its actions on membrane-bound enzymes.
    • The inhibition of lipid-enzyme interactions in the low-affinity ouabain NaK-ATPase by ethanol is linked to the observed differences in ethanol sensitivity between LS and SS mice.